Analysis for the diagnostic accuracy of PCR detection ofin companion birds.

Abstract

BACKGROUND: , a yeast-like fungus, has the potential to infect various bird species, including companion birds. Although birds infected withmay often remain asymptomatic, the infection can develop into chronic wasting gastritis and even progress to gastric cancer, highlighting the importance of early detection ofinfection. Despite direct fecal examination being a commonly used diagnostic method, the polymerase chain reaction (PCR) is anticipated to offer a higher detection rate. However, the actual diagnostic accuracy of the PCR forremains unknown. CASE DESCRIPTION: Ninety fecal samples collected from companion birds that visited or were admitted to a hospital, regardless of their stage ofdiagnosis or treatment, were subjected to PCR testing. An accuracy analysis was then performed, considering symptomatology, direct fecal testing (FT), and sequencing. The PCR test had a sensitivity of 83.33%, specificity of 95.00%, false negative rate of 16.67%, false positive rate of 5.00%, positive predictive value of 89.29%, negative predictive value of 91.94%, prevalence of 33.33%, positive likelihood ratio of 16.67, negative likelihood ratio of 0.18, and diagnostic odds ratio of 95.00. CONCLUSION: The findings suggest that the PCR forpossesses high diagnostic accuracy, with the ability to accurately identify uninfected individuals as negative. While the direct fecal examination is appropriate for routine primary screening, in cases whereis not detected by FT, the PCR may provide a more accurate and definitive diagnosis due to its high specificity.