Peer-reviewed veterinary case report
Lung bacteria in dogs with Bordetella bronchiseptica infection
By Fastrès, Aline et al.·Published in Veterinary research·2020·Department of Clinical Sciences·View original on PubMed →
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Original publication title: Analysis of the lung microbiota in dogs with Bordetella bronchiseptica infection and correlation with culture and quantitative polymerase chain reaction.
- Species:
- dog
Plain-English summary
A group of dogs with Bordetella bronchiseptica infection, which causes respiratory issues, were studied to understand the bacteria in their lungs. These infected dogs showed a higher bacterial load and less diversity in their lung bacteria compared to healthy dogs. The researchers found that the presence of Bordetella was confirmed through tests, and many of the infected dogs also had another bacteria called Mycoplasma. This study highlights how Bordetella infection can disrupt the normal bacteria in a dog's lungs, which may help vets diagnose and treat respiratory infections more effectively.
People also search for: dog coughing Bordetella treatment · dog respiratory infection symptoms · Bordetella bronchiseptica in dogs
Abstract
Infection with Bordetella bronchiseptica (Bb), a pathogen involved in canine infectious respiratory disease complex, can be confirmed using culture or qPCR. Studies about the canine lung microbiota (LM) are recent, sparse, and only one paper has been published in canine lung infection. In this study, we aimed to compare the LM between Bb infected and healthy dogs, and to correlate sequencing with culture and qPCR results. Twenty Bb infected dogs diagnosed either by qPCR and/or culture and 4 healthy dogs were included. qPCR for Mycoplasma cynos (Mc) were also available in 18 diseased and all healthy dogs. Sequencing results, obtained from bronchoalveolar lavage fluid after DNA extraction, PCR targeting the V1-V3 region of the 16S rDNA and sequencing, showed the presence of Bb in all diseased dogs, about half being co-infected with Mc. In diseased compared with healthy dogs, the β-diversity changed (P = 0.0024); bacterial richness and α-diversity were lower (P = 0.012 and 0.0061), and bacterial load higher (P = 0.004). Bb qPCR classes and culture results correlated with the abundance of Bb (r = 0.71, P < 0.001 and r = 0.70, P = 0.0022). Mc qPCR classes also correlated with the abundance of Mc (r = 0.73, P < 0.001). Bb infection induced lung dysbiosis, characterized by high bacterial load, low richness and diversity and increased abundance of Bb, compared with healthy dogs. Sequencing results highly correlate with qPCR and culture results showing that sequencing can be reliable to identify microorganisms involved in lung infectious diseases.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/32209128/