Peer-reviewed veterinary case report
How a new test measures bile acids in cats and dogs
By Weiler, Kristina et al.·Published in Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·2021·Department of Veterinary Clinical Sciences, Germany·View original on PubMed →
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Original publication title: Analytical performance and method comparison of a quantitative point-of-care immunoassay for measurement of bile acids in cats and dogs.
Plain-English summary
A study tested a new point-of-care device that measures bile acids in cats and dogs, which can help diagnose liver issues. The device was compared to standard lab tests using samples from healthy and sick pets. Results showed that the new analyzer was accurate and reliable for measuring bile acids, with strong agreement with the traditional methods. However, it had some issues with certain substances like bilirubin and lipids that could affect results. Overall, this new tool could be useful for vets to quickly assess liver function in pets.
People also search for: dog liver disease bile acids test · cat bile acids test accuracy · point-of-care analyzer for pets
Abstract
Point-of-care analyzers (POCAs) for quantitative assessment of bile acids (BAs) are scarce in veterinary medicine. We evaluated the Fuji Dri-Chem Immuno AU10V analyzer and v-BA test kit (Fujifilm) for detection of feline and canine total serum BA concentration. Results were compared with a 5th-generation assay as reference method and a 3rd-generation assay, both run on a bench-top analyzer. Analytical performance was assessed at 3 different concentration ranges, and with interferences. For method comparison, samples of 60 healthy and diseased cats and 64 dogs were included. Linearity was demonstrated for a BA concentration up to 130 µmol/L in cats (= 0.99) and 110 µmol/L in dogs (= 0.99). The analyzer showed high precision near the lower limit of quantification of 2 µmol/L reported by the manufacturer. Intra- and inter-assay coefficients of variation were < 5% for both species and all concentrations. Interferences were observed for bilirubin (800 mg/L) and lipid (4 g/L). There was excellent correlation with the reference method for feline (= 0.98) and canine samples (= 0.97), with proportional biases of 6.7% and -1.3%, respectively. However, a large bias (44.1%) was noted when the POCA was compared to the 3rd-generation assay. Total observed error was less than total allowable error at the 3 concentrations. The POCA reliably detected feline and canine BA in clinically relevant concentrations.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/33112211/