Peer-reviewed veterinary case report
Testing a human cystatin C kidney test for cat blood and urine samples
By Ghys, Liesbeth F E et al.·Published in Veterinary clinical pathology·2014·Department of Medicine and Clinical Biology of Small Animals·View original on PubMed →
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Original publication title: Analytical validation of a human particle-enhanced nephelometric assay for cystatin C measurement in feline serum and urine.
- Species:
- cat
Plain-English summary
A study found that cats with chronic kidney disease (CKD) had higher levels of a protein called cystatin C in their blood and urine compared to healthy cats. This protein can help veterinarians assess kidney function more accurately than traditional tests. The researchers validated a human test for measuring cystatin C in cats, showing it works well for feline samples. This could lead to better early detection of kidney issues in cats. More research is needed to confirm cystatin C's effectiveness as an early warning sign of kidney damage.
People also search for: cat kidney disease symptoms · cystatin C test for cats · how to detect kidney problems in cats
Abstract
BACKGROUND: In people and dogs, Cystatin C (CysC), a renal glomerular and tubular marker, seems superior to serum creatinine to estimate the glomerular filtration rate (GFR). A particle-enhanced nephelometric immunoassay is available to measure human CysC, but there are no reports in cats. OBJECTIVE: The goal of this study was the validation of the human CysC nephelometric assay with feline serum and urine, and to perform a pilot study comparing serum and urine CysC between healthy cats and cats with chronic kidney disease (CKD). METHODS: Western blot analysis was used to assess cross-reactivity between the polyclonal rabbit anti-human CysC antibody and feline CysC. Imprecision and linearity were determined for feline serum and urine CysC. Serum and urine CysC were measured in 10 healthy and 10 CKD cats. RESULTS: Cross-reactivity between the polyclonal rabbit anti-human CysC antibody and feline CysC was demonstrated. Intra- and inter-assay coefficients of variation in feline serum and urine were 1.3% and 0.4%, and 12.5%, and 4.1%, respectively. Cats with CKD had a significantly higher serum CysC concentration (1.24 [0.63-2.99] vs 0.79 [0.43-1.05] mg/L; P = .02) and urine CysC/urinary Creatinine (uCr) ratio (565.6 [0-1311] vs < 0.049/uCr mg/mol; P = .005) compared with healthy cats. CONCLUSIONS: The human nephelometric assay showed satisfactory validation results for feline CysC. Cats with CKD had a significantly higher sCysC concentration and uCysC/uCr ratio compared with healthy cats. Additional studies are necessary to evaluate CysC as an early marker of renal damage in cats.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/24773065/