Peer-reviewed veterinary case report
Analytical validation of an automated assay for ferric-reducing ability of plasma in dog serum.
- Journal:
- Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
- Year:
- 2017
- Authors:
- Rubio, Camila Peres et al.
- Affiliation:
- Department of Animal Medicine and Surgery · Spain
- Species:
- dog
Abstract
We performed analytical validation of an automated ferric-reducing ability of plasma (FRAP) assay in the serum of dogs. Intra- and interassay precision, accuracy, detection limit, and effects of hemolysis and lipemia were evaluated. Intra- and interassay coefficients of variation were <1% and <13%, respectively. The assay showed a high correlation with a FRAP assay described previously, and results were linear when serial sample dilutions were tested. The detection limit was lower than the values observed in sera from healthy dogs; decreased serum FRAP was found in dogs with leishmaniosis. Lipemia and hemolysis caused a significant increase in the results of the assay.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/28424022/