Peer-reviewed veterinary case report
Accuracy of the Vcheck cPL 2.0 test for canine pancreatic lipase
By Mendoza-White, Isabel et al.·Published in Veterinary clinical pathology·2026·Department of Small Animal Clinical Sciences, United States·View original on PubMed →
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Original publication title: Analytical Validation of an Automated Point-of-Care Immunoassay for the Measurement of Canine Pancreatic Lipase Immunoreactivity Concentration (Vcheck cPL 2.0).
- Species:
- dog
Plain-English summary
A study evaluated a new blood test for measuring pancreatic lipase levels in dogs, which helps diagnose pancreatitis. The updated test, Vcheck cPL 2.0, showed better precision than the earlier version but struggled with accuracy at very high lipase levels. While the test performed well in most cases, severe hemolysis (breakdown of red blood cells) could affect the results. Overall, this new test could be a useful tool for vets, but pet owners should be aware of its limitations, especially if their dog has severe blood issues.
People also search for: dog pancreatitis symptoms · Vcheck cPL 2.0 test accuracy · dog blood test for pancreatitis
Abstract
BACKGROUND: Pancreatic lipase assays are commonly utilized in the diagnostic approach to suspected pancreatitis in dogs. While a previously available point-of-care assay for the quantification of canine pancreatic lipase immunoreactivity (Vcheck cPL 1.0) performed sub-optimally, the updated version (Vcheck cPL 2.0) lacks independent validation. OBJECTIVE: To evaluate the analytic validity of the Vcheck cPL 2.0 assay. METHODS: Linearity was assessed via dilutional parallelism. Intra- and inter-assay variability were assessed by calculating coefficients of variation across replicates on the same day and across consecutive days, respectively. Interference studies were performed to assess the effects of bilirubin, hemoglobin, and lipid presence in test samples on assay performance. RESULTS: Linearity appeared within target parameters at pancreatic lipase concentrations up to ~800 md/dL (mean O/E ratio: 0.968 ± 0.104) but was unacceptable at pancreatic lipase concentrations > 2000 mg/dL (mean O/E ratio: 1.432 ± 0.178). Intra-assay precision (median coefficient of variation 7.2%) was acceptable; however, inter-assay precision was sub-optimal (coefficient of variation > 10%) in 50% of samples analyzed. Variable precision may result in different diagnostic interpretations. Icterus and lipemia did not impact assay results. However, hemolysis, when severe (375-500 mg/dL), may impact test results (p < 0.005). CONCLUSIONS: The Vcheck cPL 2.0 shows improved precision compared to previous versions. Linearity was unacceptable at higher pancreatic lipase concentrations and hemolysis may impact Vcheck cPL 2.0 assay results.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/42068092/