Peer-reviewed veterinary case report
Measuring fecal 3-bromotyrosine in healthy dogs and dogs
By Sattasathuchana, Panpicha et al.·Published in Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·2019·Department of Companion Animal Clinical Sciences·View original on PubMed →
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Original publication title: Analytical validation of fecal 3-bromotyrosine concentrations in healthy dogs and dogs with chronic enteropathy.
- Species:
- dog
Plain-English summary
A study found that dogs with chronic enteropathy (CE), a condition that affects their digestive system, had higher levels of a substance called 3-bromotyrosine in their feces compared to healthy dogs. Researchers developed a new method to measure this substance in dog poop, which could help in diagnosing and understanding CE better. The results showed that the average and maximum levels of 3-bromotyrosine were significantly elevated in dogs with CE, suggesting it could be a useful marker for this condition. More research is needed to see how this test can be used in veterinary practice.
People also search for: dog chronic enteropathy symptoms · dog poop test for digestive issues · elevated fecal 3-bromotyrosine in dogs
Abstract
Studies that have used serum 3-bromotyrosine (3-BrY) to investigate eosinophil activation in dogs have found elevated 3-BrY levels in clinical patients with chronic enteropathy (CE). To our knowledge, a method to measure 3-BrY concentrations in feces has not been reported. We developed and analytically validated an electron ionization gas chromatography-mass spectrometry method to measure fecal 3-BrY concentrations in dogs. The mean and maximum fecal 3-BrY concentrations in healthy dogs ( n = 40) and dogs with CE ( n = 40) over 3 consecutive days were compared. Analytical validation had a limit of blank and a limit of detection of 2.5 and 3.7 mmol/g of feces, respectively. The mean coefficients of variation for precision and reproducibility for 3-BrY were 11.2% (range: 7.5-14.2%) and 10.1% (4.8-15.2%), respectively. The ranges of observed-to-expected ratios for linearity and accuracy were 81.3-125% and 85.4-120%, respectively. The reference intervals for mean and maximum fecal 3-BrY concentrations in 40 healthy dogs were 3.7-23.0 and 3.7-37.8 mmol/g of feces. Mean and maximum fecal 3-BrY concentrations in dogs with CE were significantly higher than those of healthy dogs ( p < 0.001). Further research is warranted to determine the clinical usefulness of fecal 3-BrY concentrations in dogs with CE.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/30767618/