Peer-reviewed veterinary case report
Analyzing Necroptosis Using an RIPK1 Kinase Inactive Mouse Model of TNF Shock.
- Journal:
- Methods in molecular biology (Clifton, N.J.)
- Year:
- 2018
- Authors:
- Zelic, Matija & Kelliher, Michelle A
- Affiliation:
- Department of Molecular · United States
- Species:
- rodent
Abstract
The serine/threonine kinase RIPK1 has numerous biological and pathological functions, mediating prosurvival as well as prodeath apoptotic and necroptotic signaling pathways downstream of various receptors, including death receptors and Toll-like receptors (TLRs). RIPK1 has been implicated in various diseases, including ischemia-reperfusion injury and inflammatory bowel disease (IBD). The recent generation of RIPK1 kinase inactive mice has enabled us to genetically interrogate the role of RIPK1 kinase-mediated necroptosis in disease models. Here, we describe procedures utilizing kinase inactive Ripk1mice to analyze necroptosis induction in vitro in bone-marrow derived macrophages (BMDMs) and in vivo in a murine model of TNF-induced shock.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/30136236/