Peer-reviewed veterinary case report
Best tests to find Ancylostoma caninum hookworm eggs in dog poop
By Cringoli, Giuseppe et al.·Published in Experimental parasitology·2011·Department of Pathology and Animal Health, Italy·View original on PubMed →
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Original publication title: Ancylostoma caninum: calibration and comparison of diagnostic accuracy of flotation in tube, McMaster and FLOTAC in faecal samples of dogs.
- Species:
- dog
Plain-English summary
A study tested different methods for diagnosing hookworm (Ancylostoma caninum) in dogs by examining their stool samples. The researchers found that using a specific flotation solution (sodium chloride) and preserving the samples in a certain way (formalin 5%) led to the most accurate results. Among the techniques compared, FLOTAC was the most effective, consistently detecting more hookworm eggs than the other methods. This suggests that FLOTAC could be the best choice for diagnosing hookworm infections in dogs, helping veterinarians provide better treatment options.
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Abstract
We performed a calibration of flotation in tube, McMaster and FLOTAC to determine the optimal flotation solution (FS) and the influence of faecal preservation for the diagnosis of Ancylostoma caninum in dogs, and compared the accuracy of the three copromicroscopic techniques. Among nine different FS, sodium chloride and sodium nitrate performed best for detection and quantification of A. caninum eggs. Faecal samples, either fresh or preserved in formalin 5%, resulted in higher A. caninum egg counts, compared to frozen samples or preserved in formalin 10% or sodium acetate-acetic acid-formalin. FLOTAC consistently resulted in higher A. caninum eggs per gram of faeces (EPG) and lower coefficient of variation (CV) than McMaster and flotation in tube. The best results in terms of mean faecal egg counts (highest value, i.e. 117.0EPG) and CV (lowest value, i.e. 4.8%) were obtained with FLOTAC using sodium chloride and faecal samples preserved in formalin 5%. Our findings suggest that the FLOTAC technique should be considered for the diagnosis of A. caninum in dogs.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/21295030/