Peer-reviewed veterinary case report
Animal models in dengue.
- Journal:
- Methods in molecular biology (Clifton, N.J.)
- Year:
- 2014
- Authors:
- Plummer, Emily & Shresta, Sujan
- Affiliation:
- La Jolla Institute for Allergy and Immunology · United States
Abstract
Validation of a mouse model of dengue virus (DENV) infection relies on verification of viremia and productive replication in mouse tissues following infection. Here, we describe a quantitative assay for determining viral RNA levels in mouse serum and tissues. For the purpose of confirming DENV replication, we outline a fluorescence immunohistochemistry (FIHC) protocol for staining a nonstructural protein of DENV.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/24696349/