Peer-reviewed veterinary case report
Anisakis pegreffii: a quantitative fluorescence PCR assay for detection in situ.
- Journal:
- Experimental parasitology
- Year:
- 2011
- Authors:
- Fang, Wenzhen et al.
- Affiliation:
- School of Life Sciences · China
Abstract
To facilitate improved diagnosis and detection of the third stage larva (L3) of Anisakis pegreffii from the Minnan-Taiwan bank fishing ground in Taiwan Strait, a real-time PCR method for the detection in situ and differentiation was developed to amplify a region of the second internal transcribed spacer (ITS-2) of this parasite. The real-time PCR assay was capable of detecting 1/3 of a single L3 in 30 mg of marine fish tissue, and also exhibited a high level of specificity for A. pegreffii, no fluorescence signals were observed in other five major larval anisakid species found in commercial marine fishes caught in this fishing ground.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/21110972/