Antibody Repertoires Identify β-Tubulin as a Host Protective Parasite Antigen in Mice Infected With.

Abstract

Few studies investigate the major protein antigens targeted by the antibody diversity of infected mice with. To detect global IgG antibody specificities, sera from infected mice were immunoblotted against wholeextracts. By proteomic analysis, we were able to identify the most immunogenicproteins. We identified three major antigens as pyruvate phosphate dikinase, Hsp-85, and β-tubulin. The major protein band recognized by host IgG wasβ-tubulin. Theβ-tubulin gene was cloned, expressed in, and recombinantβ-tubulin was obtained. Infection increased IgG reactivity against recombinantβ-tubulin. A single immunization of mice with recombinantβ-tubulin increased specific IgG reactivity and induced protection againstinfection. These results indicate that repertoire analysis is a valid approach to identify antigens for vaccines against Chagas disease.