Peer-reviewed veterinary case report
Fluoroquinolone resistance and gene mutations in Pseudomonas from dogs
By Park, Youjin et al.·Published in BMC veterinary research·2020·Department of Veterinary Internal Medicine, South Korea·View original on PubMed →
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Original publication title: Antimicrobial resistance and novel mutations detected in the gyrA and parC genes of Pseudomonas aeruginosa strains isolated from companion dogs.
- Species:
- dog
Plain-English summary
A study found that many strains of Pseudomonas aeruginosa, a bacteria that can cause infections in dogs, are becoming resistant to common antibiotics like enrofloxacin and marbofloxacin. Out of 260 dogs with infections, particularly those with ear infections, a significant number had strains that were resistant to these medications. The researchers discovered specific mutations in the bacteria's genes that contribute to this resistance. This highlights the need for veterinarians to use fluoroquinolone antibiotics carefully when treating infections in dogs to ensure they remain effective.
People also search for: dog ear infection treatment · Pseudomonas aeruginosa resistance · enrofloxacin for dogs · antibiotic resistance in dogs
Abstract
BACKGROUND: Fluoroquinolone agents, such as enrofloxacin and marbofloxacin, are commonly used for pseudomonal infection in veterinary medicine. However, the rate of resistance to fluoroquinolones is rapidly increasing, according to multiple studies in various countries. Point mutations in the quinolone resistance-determining region (QRDR) are closely related to the increased fluoroquinolone resistance of Pseudomonas aeruginosa. The aim of this study was to investigate current antimicrobial susceptibility and fluoroquinolone resistance in P. aeruginosa strains isolated from dogs. The presence of point mutations in the QRDR was confirmed by gyrA and parC polymerase chain reaction and nucleotide sequencing analysis. RESULTS: A total of 84 nonduplicated P. aeruginosa strains were obtained from 228 healthy dogs (healthy group) and 260 dogs with clinical signs (infected group). Among these isolates, 38 strains from the healthy group were detected in several sample types, whereas 46 strains from the infected group were obtained mostly from dogs' ears with otitis externa (41/260, 15.8%). All strains were resistant to nalidixic acid, while some were also resistant to enrofloxacin (23/84, 27.4%), marbofloxacin (17/84, 20.2%), levofloxacin (12/84, 14.3%), or ciprofloxacin (11/84, 13.1%). Enrofloxacin resistance was significantly higher in strains from the infected group than in those from the healthy group (p < 0.05). Among the 23 fluoroquinolone-resistant strains, 8 and 4 different mutations were detected in the gyrA and parC genes, respectively. Mutations in gyrA were significantly common in the infected group (p < 0.05). Hotspots for the gyrA and parC mutations were Thr83 (34.8%, 8/23) and Pro116 (91.3%, 21/23), respectively. Double and triple mutations were also found in 5 of the strains. CONCLUSION: Novel mutations in the gyrA and parC genes were first found in P. aeruginosa isolated from companion dogs in South Korea. These findings suggest that it is important to encourage prudent use of fluoroquinolone antibiotics in canine pseudomonal infection treatment.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/32293442/