Antimicrobial susceptibility profiles of commensalisolates from chickens in Hungarian poultry farms between 2022 and 2023.

Abstract

INTRODUCTION: One of the most pressing challenges of our time is the global spread of antimicrobial resistance (AMR). Regular surveillance studies are critical for advancing collaborative efforts between animal and public health sectors, aligning with the One Health concept. METHODS: In this study, we aimed to assess the antibiotic susceptibility of commensal() strains ( = 146) isolated from largescale poultry farms in Hungary, using minimum inhibitory concentration (MIC) determinations. Additionally, PCR was employed to investigate the presence of major and minor virulence factors. RESULTS: Our findings revealed a decrease in the efficacy of penicillin-based antibiotics, which are primary choices for treating-related infections, with resistance observed in 48.3% of isolates for penicillin and 20.7% for amoxicillin. Furthermore, virulence gene analysis identified 47 strains (32.2%) carrying the majortoxin gene, one strain with thetoxin gene (0.7%), 27 strains (18.5%) with the minortoxin gene, and four strains (2.7%) with thetoxin gene. DISCUSSION: These results underscore the necessity of regular surveillance studies and highlight the significant role of commensal strains as reservoirs for sustaining resistance. Future research should include larger sample sizes to provide a more comprehensive understanding of resistance dynamics. Additionally, the genetic basis of resistance in multidrug-resistant strains should be elucidated using next-generation sequencing, enabling targeted interventions to address this growing concern.