Peer-reviewed veterinary case report
Application of a real-time PCR assay for the detection of Ascaris suum DNA in the liver of experimentally infected chickens.
- Journal:
- The Journal of veterinary medical science
- Year:
- 2021
- Authors:
- Nguyen, Yen Thi Hoang et al.
- Affiliation:
- Department of Veterinary Sciences · Japan
Abstract
This study aimed to evaluate the sampling method for the detection of Ascaris suum larval DNA in chicken livers using real-time PCR. Chickens were inoculated with A. suum eggs of a single dose (Group A) or repeatedly low doses (Group B). White spots (WSs) were continuously observed on liver from day 3 after the last infection in Group B and day 14 in Group A. In Group A, larval DNA was detected in WS lesions (78.6%) at a significantly higher rate than in the remaining tissue samples (31.3%). In conclusion, applying WS lesions to the assay improved the detection rate of A. suum DNA in chicken livers, especially in the case of a single infection.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/33658451/