Peer-reviewed veterinary case report
Application of a Tissue Clearing Method for the Analysis of Dopaminergic Axonal Projections.
- Journal:
- Methods in molecular biology (Clifton, N.J.)
- Year:
- 2021
- Authors:
- Yamauchi, Kenta et al.
- Affiliation:
- Department of Cell Biology and Neuroscience · Japan
Abstract
Parkinson's disease (PD) is a neurodegenerative disorder characterized with the progressive loss of dopaminergic (DA) neurons within the substantia nigra pars compacta (SNc). Quantitative analysis of neuronal loss including neuronal processes, axons and dendrites, would advance the understanding of the pathogenesis of PD. ScaleS, an aqueous tissue clearing method, provides stable tissue preservation while maintaining potent clearing capability, allowing quantitative three-dimensional (3D) imaging of biological tissues. In this chapter, we describe detailed procedures for 3D imaging of brain slice tissues with ScaleS technique. These include brain slice preparation, tissue clarification, chemical and immunohistochemical labeling (ChemScale and AbScale), and observation of labeled tissues using a confocal laser scanning microscope (CLSM).
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/34043200/