Peer-reviewed veterinary case report
Application of real-time RT-PCR utilising lux (light upon extension) fluorogenic primer for the rapid detection of avian influenza viruses.
- Journal:
- Acta veterinaria Hungarica
- Year:
- 2006
- Authors:
- Kiss, I et al.
- Affiliation:
- Central Veterinary Institute
- Species:
- bird
Abstract
A real-time RT-PCR assay utilising light upon extension fluorogenic primer (LUX RT-PCR) was developed for the rapid and efficient detection of avian influenza viruses (AIV). The assay detected each of the AIV isolates tested (16/16) and gave negative results with heterologous pathogens (17/17). The detection limit of the assay proved to be 10(-0.5) EID50/0.2 ml and 10(1.5) EID50/0.2 ml in allantoic fluid of virus-infected embryonated chicken eggs and in spiked chicken faeces samples, respectively. Based on its specificity, sensitivity and relative simplicity, the LUX RT-PCR assay provides a novel, rapid and cost-effective diagnostic tool for avian influenza surveillance and monitoring programs.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/17278724/