Arbovirus surveillance using FTAcards in modified CO-baited encephalitis virus surveillance traps in the Northern Territory, Australia.

Abstract

In 2016, modified CO-baited encephalitis virus surveillance (EVS) traps were evaluated for flavivirus surveillance in the Northern Territory, Australia. The traps were fitted with honey-soaked nucleic acid preservation cards (FTA) for mosquitoes to expectorate virus while feeding on the cards. Cards were tested for the presence of selected arboviruses, with two cards testing positive for Kunjin virus and Alfuy, while sentinel chickens tested in parallel also showed Kunjin virus activity at the same time. The results from the cards and vector mosquito feeding rates indicate that CO-baited EVS traps coupled with honey-baited FTAcards are an effective tool for broad-scale arbovirus surveillance.