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Peer-reviewed veterinary case report

Cold plasma kills bacteria causing dog eye infections

By Marx, Anne Helene et al.·Published in Frontiers in veterinary science·2023·Department of Small Animal Medicine and Surgery, Germany·View original on PubMed

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Original publication title: Argon cold atmospheric plasma eradicates pathogensthat are commonly associated with canine bacterial keratitis.

Species:
dog

Plain-English summary

A study found that cold atmospheric plasma (CAP) can effectively kill bacteria linked to eye infections in dogs, specifically those with bacterial keratitis. Researchers tested this treatment on various bacterial strains taken from infected dogs and discovered that a 5-minute application of CAP was the most effective at reducing bacterial growth. This method showed significant promise in treating these infections, suggesting it could be a useful option for veterinarians dealing with canine eye problems.

People also search for: dog eye infection treatment · canine bacterial keratitis · cold plasma therapy for dogs

Abstract

PURPOSE: To investigate the antimicrobial effect of cold atmospheric plasma (CAP) on pathogens associated with canine bacterial keratitis. MATERIALS AND METHODS: ,, andstrains, which were obtained from dogs with infectious keratitis, were subjected to testing. For each species, four isolates and a reference strain were cultivated on Columbia sheep blood agar and treated with the kiNPen Vetplasma pen from Neoplas GmbH, Greifswald, Germany. Various continuous treatment durations (0.5, 2, and 5 min) were applied, along with a 0.5-min treatment repeated four times at short intervals. These treatments were conducted at distances of 3 and 18 mm between the agar surface and the pen. RESULTS: CAP treatment reduced bacterial growth in all three species. The most effective treatment duration was 5 min at 3 mm distance, resulting in inhibition zones ranging from 19 to 22 mm for, 26-45 mm forand an overall reduction of bacterial growth for. Inhibition zones were smaller with decreasing treatment duration and larger distance. Treatment times of 30 s repeated four times and 2 min showed comparable results. Treatment with argon alone did not lead to visible reduction of bacterial growth. CONCLUSION: Argon cold atmospheric plasma demonstrated a potentantimicrobial effect onandstrains with the latter showing the highest sensitivity.

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Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/38264471/