Asiaticoside alleviated NAFLD by activating Nrf2 and inhibiting the NF-κB pathway.

Abstract

BACKGROUND: Nonalcoholic fatty liver disease (NAFLD) is a globally prevalent clinical problem of chronic liver disorder whose main pathogenic mechanisms are hepatic lipid accumulation, oxidative stress, and inflammatory reactions. Asiaticoside (Asi) is a compound derived from Centella asiatica, possesses antioxidant, antiphlogistic, antifibrotic, as well as wound healing properties. PURPOSE: The aim of this study was to investigate the effects of Asi on NAFLD and its potential mechanisms. STUDY DESIGN: Two versions of experimental models were constructed using free fatty acids (FFAs)-stimulated HepG2 cells along with high-fat diet (HFD)-incited NAFLD in mice. METHODS: The pivotal action of Nrf2 was then explored using Ml-385 or Nrf2mice. RESULTS: The results indicated that Asi activated the Nrf2 to alleviate oxidative stress, inhibited the NF-κB to reduce the inflammatory response, and notably decreased lipid droplets and alleviated steatosis. CONCLUSION: In conclusion, Asi demonstrates a potential to activate Nrf2 as well as inhibit the NF-κB pathway, there alleviate NAFLD.