Association of three beta-defensin gene (AvBD4, AvBD5, AvBD14) polymorphisms with carrier-state susceptibility to salmonella in chickens.

Abstract

1. Chicken salmonellosis is a common zoonotic infectious disease transmitted both vertically and horizontally. Avian beta-defensins (gallinacins) play an important role in the innate defence of the host and provide broad-spectrum immunity against multiple pathogens. 2. To detect the relationship between immune genes and salmonella carrier status and susceptibility to salmonellosis in chickens, polymorphisms with carrier-state susceptibility to salmonella and, hence, developing salmonellosis, were investigated in three avian beta-defensin genesandin a Chinese local chicken breed, based on a case-control study. 3. Fifteen, twenty and nineteen SNPs were found inand, respectively. Among the 54 total SNPs, four resulted in non-synonymous substitution of amino acid changes. Five SNPs inand four SNPs inwere significantly associated with salmonellosis susceptibility (P&#xa0;<&#xa0;0.05). Using the PHASE program, thirteen, ten and twelve major haplotypes were constructed inand. Logistic regression analysis revealed that five haplotypes inand six haplotypes inwere significantly associated with salmonellosis susceptibility, but no significant haplotype inwas detected. A total of six strongly susceptible haplotypes with odds ratio (OR) values greater than 2.0 and four strongly resistant haplotypes with OR value less than 0.5 were revealed in the three genes examined. 4. These results suggested that theandgenes may play an important role in the susceptibility to salmonellosis in chickens.