Association ofandgenes with milk production traits in Chinese Holstein cows: polymorphism and functional validation analysis.

Abstract

Milk production traits play an important role in dairy cattle breeding, and single nucleotide polymorphisms can be used as effective molecular markers for milk production trait marker-assisted breeding in dairy cattle. Based on the results of the preliminary GWAS, candidate genesandassociated with milk production traits were screened. In this study, the aim was to screen and characterize the SNPs ofandgenes about milk production traits. Two SNPs and one haplotype block of thegene and four SNPs and one haplotype block of thegene were obtained by amplification, sequencing and association analysis, and all six SNPs were located in the exon region. Association analysis showed that all six SNPs were significantly associated with milk protein percentage. Linkage disequilibrium analysis showed that 2 SNPs of(g. 36,700,265 C > T and g. 36,693,596 C > A) constituted a haplotype that correlated with milk protein percentage, and the dominant haplotype was H2H2, which was CCTT. 4 SNPs of(g. 37,342,705 C > A, g. 37,343,379 G > T, g. 37,374,314 C > A and g. 37,377,857 G > A) constituted a haplotype associated with milk protein percentage, 305-days milk protein yield and 305 days milk yield. Tissue expression profiling results revealed thatandhad the highest expression in mammary tissue. Interference withandinhibited the proliferation of Bovine mammary epithelial cells. (BMECs), down-regulated the expression of,and, up-regulated the expression ofand, and promoted apoptosis. Reduced triglyceride synthesis in BMECs, down-regulated the expression of,,, and.andare involved in the synthesis of milk proteins, and interfering withanddecreased the secretion of-casein,-casein, and αs1-casein, as well as up-regulated theandexpression. The above results indicate that the SNP loci ofandcan be used as potential molecular markers to improve milk production traits in dairy cows, laying the foundation for marker-assisted selection. It also proves thatandcan be used as candidate key genes for milk production traits in dairy cows, providing new insights into the physiological mechanisms of lactation regulation in dairy cows.