Peer-reviewed veterinary case report
Detecting canine distemper virus in dog urine and white blood cells
By F.J. Negrão et al.·Published in Arquivo Brasileiro de Medicina Veterinária e Zootecnia·2007·View original on DOAJ →
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Original publication title: Avaliação da urina e de leucócitos como amostras biológicas para a detecção ante mortem do vírus da cinomose canina por RT-PCR em cães naturalmente infectados Evaluation of the urine and leucocytes as biological samples for ante mortem detection of canine distemper virus by RT-PCR assay in naturally infected dogs
- Species:
- dog
Plain-English summary
A group of 188 dogs showing symptoms of canine distemper, such as fever, coughing, or neurological issues, were tested to see if urine or white blood cells could be used to detect the virus before death. The tests found the virus in about two-thirds of the dogs, with some having it in both urine and blood cells, while others only had it in one type of sample. This shows that relying on just one type of sample might miss some cases of the virus, which can lead to false-negative results.
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Abstract
<abstract language="eng">Urine and leucocytes were comparatively evaluated as clinical samples for ante mortem detection of the canine distemper virus (CDV) by a reverse transcription-polymerase chain reaction (RT-PCR) assay. One hundred and eighty eight dogs with clinical symptoms of distemper, were distributed in three groups. The group A was constituted of 93 dogs with systemic signs of distemper; the group B by 11 dogs with neurological signs, and the group C by 84 dogs that presented simultaneously systemic and neurological signs. In 66.5% (125/188) of the dogs was amplified an amplicon with 287 base pair of the CDV nucleoprotein gene. In 60.8% (76/125) of the animals the CDV was detected simultaneously in the urine and leucocytes, and in 39.2% (49/125) of the dogs just a type of clinical sample (urine: n=37; leucocytes: n=12) was positive. These results demonstrate that the different forms of clinical distemper disease can hinder the choice of only one type of clinical sample to carry out the ante mortem etiological diagnosis of CDV infection, and false-negative results can be generated.
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Search related cases →Original publication on DOAJ: https://doi.org/10.1590/S0102-09352007000100042