BA5 Attenuates Carbon Tetrachloride-Induced Hepatotoxicity in Mice.

Abstract

The association between liver disease and gut microbiota is being widely investigated. Probiotics, such as, are among the most notable microbiomes examined in this study.shows potential for promoting growth and effectively regulating gut microbiota, though its mechanism of action remains unclear.The early gavage administration ofBA5 conferred protection against liver injury in carbon tetrachloride (CCl)-induced mice. Growth parameters (body weight and organ index), serum biochemical markers (ALT, AST, T-SOD, MDA, GSH-Px, and T-AOC), liver and jejunum histopathology, and gut microbiota composition were comprehensively evaluated.BA5 supplementation restored serum T-AOC, T-SOD, and GSH-Px levels and attenuated CCl-induced increases in ALT, AST, and MDA, suggesting potent anti-oxidant properties. Furthermore, histopathologic assessment showed that CCl-induced mice developed acute liver injury and intestinal villi were destroyed, while the BA5 group restored the pathological changes in the tissues to the normal group level. In addition, immunohistochemical staining revealed that BA5 increased the expression level of Claudin-1 which was a key biomarker for assessing the integrity of epithelial/endothelial barriers. Regarding gut microbiota, BA5 significantly enhanced the abundance of beneficial bacteria () and decreased the abundance of hazardous bacteria (,,, and) caused by CCl. Notably, BA5 alone remarkably increased gut microbial diversity compared with that of the Control group.Overall, these findings suggest that BA5 holds promise as a potential therapeutic agent for alleviating CCl-induced acute liver injury in mice by mitigating oxidative stress and modulating gut microbiota.