Peer-reviewed veterinary case report
Babesia canis vogeli: a novel PCR for its detection in dogs in Australia.
- Journal:
- Experimental parasitology
- Year:
- 2006
- Authors:
- Martin, Anthony R et al.
- Affiliation:
- School of Environmental and Life Sciences · Australia
- Species:
- dog
Abstract
Babesia canis vogeli is known to cause disease in dogs in Australia, and the rapid detection of various subspecies would enable effective treatment and management. A 21 bp oligonucleotide, "Bab-f" was proposed for the production of larger PCR products with high species specificity that would enable effective sequence analyses to yield subspecies identification. The new forward primer when paired with a previously reported "Babesia common" reverse primer generated a 394 bp product which was successfully amplified and provided subspecies differentiation by sequence analyses. Specificity and sensitivity were reported at 100% on a cohort of 55 dogs.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/16256109/