Peer-reviewed veterinary case report
Baculovirus expression and diagnostic utility of the glycoprotein E of bovine herpesvirus-1.1 Egyptian strain "Abu-Hammad".
- Journal:
- Journal of virological methods
- Year:
- 2013
- Authors:
- El-Kholy, Alaa A et al.
- Affiliation:
- Veterinary Serum and Vaccine Res. Instit.
Plain-English summary
Researchers created a new test using a specific protein from a strain of bovine herpesvirus (BoHV-1) found in Egypt. This test, called an indirect gE-ELISA, helps detect antibodies in cattle that have been infected with the virus. They found that this new test worked well when compared to other standard methods, showing it could reliably identify infected cattle versus those that were just vaccinated. The test is promising because it is both accurate and cost-effective for monitoring cattle health. Overall, the new test appears to be a successful tool for diagnosing infections in cattle.
Abstract
A recombinant baculovirus construct expressing glycoprotein E (gE) of the Egyptian BoHV-1.1 Abu-Hammad strain (rBac/gE-AbuH) was generated and characterized. The recombinant gE (rgE) secreting protein in culture medium of infected insect cells was used as a coating antigen in an indirect enzyme-linked immunosorbent assay (ELISA) to test its utility for detection of antibody against gE of BoHV-1. Indirect gE-ELISA was compared to standard virus neutralization test and commercial blocking gE-ELISA for detection of anti-gE antibody in a panel of bovine sera. Antibody titers estimated by both ELISAs were closely correlated with those determined by virus neutralization test. In conclusion, the developed indirect gE-ELISA was a reliable candidate for inexpensive detection of anti-gE antibody in control and experimental bovine sera with high specificity and sensitivity. Moreover, it emphasized the diagnostic utility of gE based ELISAs to distinguish cattle infected with BoHV-1 from those vaccinated with the gE negative mutants.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/23557665/