Benefits of heat-killedon growth performance, nutrient digestibility, antioxidant status, immunity, and cecal microbiota of rabbits.

Abstract

INTRODUCTION: Heat-killed probiotics, as a type of inactivated beneficial microorganisms, possess an extended shelf life and broader adaptability compared to their live counterparts. This study aimed to investigate the impact of heat-killed(, LA) - a deactivated probiotic on the growth performance, digestibility, antioxidant status, immunity and cecal microbiota of rabbits. METHODS: Two hundred weaned Hyla rabbits were randomly allocated into five equal groups (CON, L, L, L, and L). Over a 28-day period, the rabbits were fed basal diets supplemented with 0, 200, 400, 600, and 800 mg/kg of heat-killed LA, respectively. RESULTS: Results revealed a significant reduction in the feed-to-gain ratio (F/G) in the Land Lgroups (&#x2009;<&#x2009;0.05). Additionally, the Lgroup exhibited significantly higher apparent digestibility of crude fiber (CF) and crude protein (CP) (&#x2009;<&#x2009;0.05). Regarding digestive enzyme activities, enhanced trypsin and fibrinase activities were observed in the Land Lgroups (&#x2009;<&#x2009;0.05). Concerning the regulation of the body's antioxidant status, the Lgroup demonstrated elevated levels of superoxide dismutase (SOD) and total antioxidant capacity (T-AOC) in both serum and ileal tissue (&#x2009;<&#x2009;0.05). In terms of immune capacity modulation, serum tumor necrosis factor-&#x3b1; (TNF-&#x3b1;) levels were significantly lower in the Land Lgroups (&#x2009;<&#x2009;0.05), while immunoglobulin A (IgA) and immunoglobulin M (IgM) levels were higher (&#x2009;<&#x2009;0.05). Additionally, the Lgroup exhibited a substantial increase in secretory immunoglobulin A (SIgA) levels in the intestinal mucosa (&#x2009;<&#x2009;0.05). In comparison to the CON group, the Lgroup exhibited a significant increase in the relative abundance ofandin the cecum (&#x2009;<&#x2009;0.05).demonstrated a positive correlation with SIgA (&#x2009;<&#x2009;0.05), IgM (&#x2009;<&#x2009;0.01), and Glutathione peroxidase (GSH-Px) (&#x2009;<&#x2009;0.05), while displaying a negative correlation with TNF-&#x3b1; levels (&#x2009;<&#x2009;0.05). Concurrently, Alistipes exhibited positive correlations with IgA (&#x2009;<&#x2009;0.05), IgM (&#x2009;<&#x2009;0.05), SIgA (&#x2009;<&#x2009;0.01), GSH-Px (&#x2009;<&#x2009;0.05), SOD (&#x2009;<&#x2009;0.05), and T-AOC (&#x2009;<&#x2009;0.01), and a negative correlation with TNF-&#x3b1; (&#x2009;<&#x2009;0.05). DISCUSSION: In conclusion, the dietary incorporation of 600 mg/kg and 800 mg/kg of heat-killed LA positively influenced the growth performance, nutrient digestibility, antioxidant status, immune capacity and cecal microbiota of rabbits. This highlights the potential benefits of utilizing heat-killed probiotics in animal nutrition.