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Peer-reviewed veterinary case report

BHV-1 induced oxidative stress contributes to mitochondrial dysfunction in MDBK cells.

Journal:
Veterinary research
Year:
2016
Authors:
Zhu, Liqian et al.
Affiliation:
College of Veterinary Medicine and Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses · China

Abstract

The levels of cellular reactive oxygen species (ROS) and ATP as well as the mitochondrial membrane potential (MMP) in response to bovine herpesvirus 1 (BHV-1) infection of MDBK cells were measured, respectively. BHV-1 infection increased ROS production which depended on viral entry, and de novo protein expression and/or DNA replication. Vice versa, excessive ROS was required for efficient viral replication. Levels of both ATP and MMP were significantly decreased after BHV-1 infection. Interestingly, the loss of MMP was ameliorated by ROS depression. Collectively, ROS dependent mitochondrial damage and ultimately disruption of energy metabolism (ATP depletion) are a potential pathogenic mechanism for BHV-1 infection.

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Original publication: https://pubmed.ncbi.nlm.nih.gov/27000063/