Biocidal effects of a wipe-down procedure using common veterinary cleansers on microbial burden within working canine exterior coats.

Abstract

INTRODUCTION: Recent work demonstrating reduction of aerosolized contamination via a wipe-down procedure using common veterinary antiseptics offers promise regarding health concerns associated with cross-contamination from working canines to humans. While mechanical reduction can be achieved via a wipe-down procedure, the biocidal impact on flora within the exterior coat is unknown. METHODOLOGY: This study assessed the biocidal impact of antiseptics on the exterior bacterial community of the canine. Lint-free towels were saturated with 2% chlorhexidine gluconate scrub, or 7.5% povidone-iodine scrub diluted at a 1:4 ratio. Treatments were rotated across the dorsal aspect of kennel housed Foxhounds (&#x2009;=&#x2009;30). Sterile swabs were collected in triplicate prior to, and following wipe down, stored in Amies solution at 4&#xb0;C, plated onto nutrient agar and reduction in colony forming units (CFU) was measured across both treatments. Statistical analysis utilizing PROC GLM examined effects of treatment (&#x2009;&#x2264;&#x2009;0.05). Molecular analysis of the 16S rRNA gene was completed for 3 hounds. RESULTS: Reduction in CFU was measured (&#x2009;<&#x2009;0.001) for both antiseptics. Qualitative molecular data indicated that both antiseptics had a biocidal effect on the dominant microbial community on the exterior coat with gram-positive, spore-forming taxa predominating post-treatment. CONCLUSION: Effective wipe-down strategies using common veterinary cleansers should be further investigated and incorporated to safeguard working canine health and prevent cross-contamination of human personnel.