Biodegradation of bovine spongiform encephalopathy prions in compost.

Abstract

To reduce the transmission risk of bovine spongiform encephalopathy prions (PrP^BSE), specified risk materials (SRM) that can harbour PrP^BSE are prevented from entering the feed and food chains. As composting is one approach to disposing of SRM, we investigated the inactivation of PrP^BSE in lab-scale composters over 28 days and in bin composters over 106-120 days. Lab-scale composting was conducted using 45 kg of feedlot manure with and without chicken feathers. Based on protein misfolding cyclic amplification (PMCA), after 28 days of composting, PrP^BSE seeding activity was reduced by 3-4 log₁₀ with feathers and 3 log₁₀ without. Bin composters were constructed using ~ 2200 kg feedlot manure and repeated in 2017 and 2018. PMCA results showed that seeding activity of PrP^BSE was reduced by 1-2 log₁₀ in the centre, but only by 1 log₁₀ in the bottom of bin composters. Subsequent assessment by transgenic (Tgbov XV) mouse bioassay confirmed a similar reduction in PrP^BSE infectivity. Enrichment for proteolytic microorganisms through the addition of feathers to compost could enhance PrP^BSE degradation. In addition to temperature, other factors including varying concentrations of PrP^BSE and the nature of proteolytic microbial populations may be responsible for differential degradation of PrP^BSE during composting.