Peer-reviewed veterinary case report
Blood and urine tests to check liver oxidative stress in dogs
By Barry-Heffernan, Caitlin et al.·Published in Journal of veterinary internal medicine·2019·BluePearl Veterinary Partners, United States·View original on PubMed →
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Original publication title: Biomarkers of oxidative stress as an assessment of the redox status of the liver in dogs.
- Species:
- dog
Plain-English summary
A group of 34 dogs with liver issues underwent biopsies to assess their liver health and oxidative stress levels. The study measured various markers in their blood and urine, including glutathione (a key antioxidant) and vitamin E. It found that the levels of glutathione in the blood did not reflect the levels in the liver, and dogs with liver problems showed higher signs of oxidative stress compared to healthy dogs. Most of the dogs received treatments like antioxidants or special liver diets, but the study did not specify the outcomes for individual dogs.
People also search for: dog liver disease symptoms · antioxidants for dogs with liver problems · liver biopsy in dogs · signs of oxidative stress in dogs
Abstract
BACKGROUND: Oxidative stress is associated with a diverse group of liver disorders across species. OBJECTIVES: Determine whether glutathione (GSH) concentration in plasma and red blood cells correlates with liver GSH concentration in dogs and evaluate whether other markers of systemic oxidative stress, plasma vitamin E and urine 8-isoprostanes/creatinine (F-IsoPs/Cr) concentrations, correlate with liver GSH. ANIMALS: Thirty-four client-owned dogs undergoing clinically indicated liver biopsy and 15 healthy control dogs. METHODS: Prospective, observational cross-sectional study. Urine and blood were collected before liver biopsy. Plasma, erythrocyte, and liver GSH were measured using high performance liquid chromatography (HPLC); vitamin E was measured by HPLC, and F-IsoPs/Cr was measured by gas chromatography/mass spectrometry. RESULTS: All dogs were treated at the discretion of the attending clinician (24/34 received antioxidants; 4/34 fed therapeutic liver diet), which included dogs with primary or secondary liver disease (inflammatory (n = 21), metabolic (n = 9), vascular (n = 2), and neoplastic (n = 2)). Median GSH concentrations in plasma, erythrocyte, and liver were 0.18 mg/dL (range 0.14 to 0.56 mg/dL), 56.7 mg/dL (18.3 to 79.2 mg/dL), and 181 mg/dL (39.9 to 527 mg/dL), respectively. No significant correlations were found between liver GSH and erythrocyte GSH, plasma GSH, vitamin E, or F-IsoPs/Cr. Dogs undergoing clinically indicated liver biopsy had significantly higher urine F-IsoPs/Cr than did healthy controls (5.89 vs 2.98 ng/mg; P < .0001). CONCLUSIONS AND CLINICAL IMPORTANCE: Erythrocyte and plasma GSH are not indicative of liver GSH concentration in dogs. In addition, dogs undergoing clinically indicated liver biopsy have evidence of increased systemic oxidative stress compared to healthy controls.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/30758875/