Peer-reviewed veterinary case report
How canine parvovirus infection is diagnosed using blood and stool
By Palanci, Pinar & Tekelioglu, Bilge Kaan·Published in Cellular and molecular biology (Noisy-le-Grand, France)·2023·Department of Biotechnology·View original on PubMed →
PetCaseFinder translated the abstract of this peer-reviewed paper into plain English so pet owners can read it. We do not publish original research — every detail traces back to the citation above. How we work →
Original publication title: Biotechnological and virological analysis of canine parvovirus infections by C-reactive protein levels, serological, hematological and molecular techniques.
- Species:
- dog
Plain-English summary
A group of unvaccinated puppies under 1 year old showed signs of illness, including diarrhea and lethargy, and were diagnosed with canine parvovirus (CPV). Testing revealed that over half of the samples were positive for CPV using various methods, including stool tests and blood tests. While the puppies had low white blood cell counts and other blood abnormalities, these findings were not specific to CPV. Unfortunately, CPV can be severe and even fatal, so early diagnosis and treatment are crucial. Treatment typically involves supportive care, such as fluids and medications to manage symptoms.
People also search for: puppy diarrhea treatment · canine parvovirus symptoms · unvaccinated dog illness · CPV diagnosis tests
Abstract
The study aims to approach Canine Parvovirus (CPV) diagnosis using multi-method biotechnological techniques including molecular, serological, and hematological analyses. CPVs are causing severe global viral diseases with high dog mortality. Samples were taken from 52 unvaccinated dogs exhibiting symptoms between 2020 and 2021. These included stool, blood, serum, and patient data. CPV genomic DNA was extracted from fresh stools, with DNA concentration and purity measured using Nano drop-spectrophotometry. CPV genomic DNA was detected via RT-PCR in 29 samples (55.8%), CPV IgM-Ab and IgG-Ab were detected in the sera through ELISA in 27 samples (51.9%), and Canine parvovirus antigens were identified in the stool samples by immunochromatography in 20 samples (38.5%). Utilizing canine-specific quantitative ELISA kits, the average level of serum C-reactive protein (CRP) was determined to be 4.66 g/L (with a range of 3.27 to 6.05 g/L). Hematological analysis revealed lymphopenia in 89.6%, leucopenia in 44.8%, anemia in 68.9%, and low hematocrit in 82.8%. All the dogs examined were under 1 year of age, among which 21 (72.4%) were up to 3 months old, and 8 (27.6%) were up to 6 months old, testing positive for CPV. The highest CPV positivity, at 93.1% (n=27), was observed among dogs with outdoor access. The results indicated that hematological parameters and CRP alone were not specific for CPV diagnosis, but provided valuable data for prognosis and differential diagnosis. No significant differences were observed in RT-PCR and ELISA results. However, a noticeable reduction in positivity rates was evident in lateral immunochromatographic viral antigen detection in stool.
Find similar cases for your pet
PetCaseFinder finds other peer-reviewed reports of pets with the same symptoms, plus a plain-English summary of what was tried across them.
Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/38163255/