Peer-reviewed veterinary case report
How antibiotics affect inflammatory markers in dog skin cells
By White, Amelia G et al.·Published in Veterinary dermatology·2014·College of Veterinary Medicine, United States·View original on PubMed →
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Original publication title: Canine progenitor epidermal keratinocytes express various inflammatory markers, including interleukin-8 and CD40, which are affected by certain antibiotics.
- Species:
- dog
Plain-English summary
A study found that certain antibiotics can affect inflammation in skin cells from dogs. When exposed to antibiotics like enrofloxacin and cefalexin, these skin cells showed increased levels of a protein called interleukin-8, which is involved in inflammation. This suggests that while treating bacterial skin infections in dogs, some antibiotics might also trigger inflammation in the skin. Pet owners should discuss with their veterinarian the best treatment options for their dog's skin infections to avoid potential side effects.
People also search for: dog skin infection treatment · antibiotics for dog skin problems · inflammation in dog skin
Abstract
BACKGROUND: Bacterial skin infections are common in dogs and humans. Keratinocytes have phenotypic features of nonprofessional antigen-presenting cells and express various cytokines. However, little is known about the effects of antibiotics on inflammatory markers in canine keratinocytes. HYPOTHESIS/OBJECTIVES: To investigate inflammatory markers in canine progenitor epidermal keratinocytes (CPEKs) and to determine the effects of selected antibiotics on these markers. METHODS: The CPEKs were exposed for 2-24 h to three concentrations of amoxicillin, cefalexin, sulfadimethoxine, sulfamethoxazole (or its nitroso metabolite), amikacin or enrofloxacin. Enzyme-linked immunosorbent assay (ELISA) and immunocytochemistry were used to detect major histocompatibility complex (MHC) II. CD40 and CXCR1 [interleukin (IL)-8 receptor] were detected using ELISA. Secreted cytokines/chemokines were quantified using a multiplex kit. RESULTS: No MHC II protein was detected. CD40 protein was found at 24 h, with levels being significantly increased by enrofloxacin. The CPEKs secreted no detectable monocyte chemotactic protein-1; undetectable to low (picogram per millilitre range) concentrations of IL-6, IL-7, IL-10, IL-15, tumour necrosis factor-α, interferon-γ and granulocyte-macrophage colony-stimulating factor; and high (nanogram per millilitre range) concentrations of IL-8. Levels of IL-8 increased over 24 h following cell proliferation. They were significantly increased by enrofloxacin after 8 h, and by cefalexin, sulfadimethoxine, sulfamethoxazole, its nitroso metabolite and enrofloxacin after 24 h. The CPEKs expressed CXCR1. CONCLUSIONS AND CLINICAL IMPORTANCE: Canine progenitor epidermal keratinocytes express various inflammatory proteins, with expression profiles being affected by certain antibiotics. This supports previous work showing keratinocytes to be mediators of inflammation and demonstrates the potential pro-inflammatory effects of certain antibiotics in the skin.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/25139312/