Carbapenemase-producing Enterobacterales strains causing infections in companion animals-Portugal.

Abstract

UNLABELLED: An increase incarbapenem-resistant human nosocomial strains is occurring in Europe, namely with theandgenes. We determined the prevalence of carbapenemase-producing Enterobacterales clinical strains in companion animals in Portugal and characterized their mobile genetic elements. Susceptibility data of a consecutive collection of 977 Enterobacterales clinical strains from a Portuguese private veterinary diagnostic laboratory were evaluated (January-December 2020). Additional phenotypical and genotypical assays were performed in a subset of 261 strains with a resistant phenotype. Whole-genome sequencing was performed for carbapenemase-producing strains. The frequency of carbapenemase-producing Enterobacterales clinical strains in companion animals in Portugal was 0.51% (= 5/977). Thus, five strains were characterized: (i) one OXA-181-producingST273, (ii) two KPC-3-producingST147; (iii) one KPC-3-producingST392; and (iv) one OXA-48-producingST127. Thegene was located on transposon Tnon IncFIA type plasmid for theST147 strains and on a IncN-type plasmid for theST392 strain, whilegene was located on an IncX3 plasmid. Allassembled plasmids and plasmid-encoded transposons harboring carbapenemase genes were homologous to those previously described in the human healthcare. No plasmid replicons were detected on the OXA-48-producingST127. The dissemination of carbapenem resistance is occurring horizontallyplasmid spreading from the human high burden carbapenem resistance setting to the companion animal sector. Furthermore, companion animals may act as reservoirs of carbapenem resistance. Implementation of carbapenemase detection methods in routine clinical veterinary microbiology is urgently needed. IMPORTANCE: This is the first study on the prevalence of carbapenemase-producing Enterobacterales (CPE) clinical strains from companion animals in Portugal. Despite the generally low prevalence of CPE in companion animals, it is imperative for veterinary diagnostic laboratories to employ diagnostic methods for carbapenemase detection. The resemblance found in the mobile genetic elements transporting carbapenemase genes between veterinary medicine and human medicine implies a potential circulation within a One Health framework.