Carbon source utilisation and evaluation of the Biolog system in the identification of.

Abstract

Sixty-eightstrains were isolated from porcine acute pleuropneumonia cases from different parts of Hungary between 2000 and 2014. A total of 41 isolates were identified asbio-type I and 27 strains as biotype II based on cultural, morphological and biochemical characteristics. The aim of this study was to evaluate metabolic fingerprinting in the species-level identification ofisolates. Utilisation of carbon sources by these field isolates and six reference strains was characterised by the Biolog system (GN2 Microplate, MicroLog3 Version 4.20.05 software). Twenty-nine field strains were correctly identified by the Biolog system as, 36 strains as, two strains asand one strain asafter 24 h of incubation. Among the sixreference strains the Biolog system identified one strain as, four asand one asThere was no correlation between biotypes and serotypes ofand the carbon source utilisation pattern and species identification by the Biolog system. our data indicate that the efficacy of the Biolog system used here could be improved by including phenotypes of morestrains representing a wider geographical occurrence into the database.