Peer-reviewed veterinary case report
Cardiomyocyte-Specific FoxO1 Knockout Mice as Tools to Assess Cardiac Hypertrophy and Key Experimental Considerations Using Cre-loxP.
- Journal:
- Methods in molecular biology (Clifton, N.J.)
- Year:
- 2025
- Authors:
- Weeks, Kate L & McMullen, Julie R
- Affiliation:
- Department of Anatomy and Physiology · Australia
- Species:
- rodent
Abstract
The generation of tissue-specific mouse models has provided a powerful strategy to understand the role of genes in specific tissues/cells of interest under control/basal conditions and in response to physiological and pathological stimuli. Here we describe the generation of cardiomyocyte-specific FoxO1 knockout mice using Cre-loxP technology to examine the role of FoxO1 for the induction of heart enlargement (cardiac hypertrophy) in settings of health and disease. We highlight breeding strategies for generating tissue-specific mouse models and key experimental considerations during characterization.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/39565579/