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Peer-reviewed veterinary case report

Changes in Hemoglobin Isoforms in the Peripheral Blood of Rats with Experimental Posthemorrhagic Anemia.

Journal:
Bulletin of experimental biology and medicine
Year:
2021
Authors:
Yushkov, B G et al.
Affiliation:
Institute of Immunology and Physiology
Species:
rodent

Abstract

Six hemoglobin isoforms were identified in the peripheral blood of male Wistar rats by PAAG electrophoresis. Erythrocytes can be separated into 6 fractions by fractional centrifugation; the fractions differ by the content of reticulocytes and cells with fetal hemoglobin. Cells in each fraction contain one light and one heavy hemoglobin isoforms, and their combination is specific to each fraction. Changes in the hemoglobin isoforms in the whole blood in case of blood loss can be associated with changes in physical and chemical properties of circulating erythrocytes, as well as with the formation of various cell populations resulting from activated erythropoiesis.

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Original publication: https://pubmed.ncbi.nlm.nih.gov/34542743/