Peer-reviewed veterinary case report
Characterisation of Chlamydia psittaci isolated from a horse.
- Journal:
- Veterinary microbiology
- Year:
- 1990
- Authors:
- Wills, J M et al.
- Affiliation:
- Department of Medical Microbiology
Plain-English summary
This study focuses on a strain of Chlamydia psittaci, a type of bacteria, that was taken from a horse experiencing a runny nose. The researchers were able to grow this bacteria in a lab setting using special cells and confirmed its identity through various tests, including looking at it under an electron microscope. They found that this strain grew slowly and produced a low amount of the bacteria during its growth cycle. Overall, the findings help in understanding this specific strain of bacteria in horses.
Abstract
This paper describes the isolation and characterisation of a strain of Chlamydia psittaci obtained from a nasal swab taken from a horse with serous nasal discharge. Initial isolation was achieved in cycloheximide-treated McCoy cell monolayers. Chlamydial inclusions stained by immunofluorescence either with a rabbit antiserum raised against C. psittaci or with a monoclonal antibody directed against the genus-specific lipopolysaccharide antigen were single and compact. They did not stain with iodine or with a monoclonal antibody reactive against Chlamydia trachomatis. The agent was re-isolated in the yolk sacs of embryonated hens eggs and designated N16. Identification of the agent was confirmed by electron microscopy. Unique plasmid DNA was prepared from a purified suspension of chlamydial elementary bodies (EBs), and analysed by electrophoresis through 1.0% agarose gels stained by ethidium bromide. This strain of C. psittaci grew relatively slowly in cycloheximide-treated McCoy cells, and the yield of elementary bodies during the course of one growth cycle was relatively low.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/2219661/