Peer-reviewed veterinary case report
Characterization of a canine long-term T cell line (DLC 01) established from a dog with Sézary syndrome and producing retroviral particles.
- Journal:
- Leukemia
- Year:
- 1999
- Authors:
- Ghernati, I et al.
- Affiliation:
- Establissement de Transfusion Sanguine · France
- Species:
- dog
Plain-English summary
Researchers have created a special cell line called DLC 01 from a dog with Sézary syndrome, a type of skin cancer that affects T cells. This cell line has specific characteristics that make it different from similar cells in humans, which could help scientists understand how these cells work. The DLC 01 cells can produce retroviral particles, which are tiny infectious agents, after being grown for a certain number of times in the lab or treated with a specific chemical. This is the first time a dog’s lymphoid cells have been found to grow and produce a retrovirus on their own, showing similarities to viruses found in cats and mice. Overall, this research provides a new tool for studying T cell behavior and viral interactions in dogs.
Abstract
The canine DLC 01 cell line derives from a lymph node of a dog with Sézary syndrome. The DLC 01 cell phenotype is CD4-, CD8+, CD45+, DQ+, similar to that of original cells after treatment with dimethylsulfoxide or phorbol myristate. Canine cutaneous T cell lymphoma are usually CD4-, CD8+ in contrast to their human counterparts which are CD4+, CD8-. Therefore, the DLC 01 cell line appears to be a unique model to study the mechanism of all surface molecule expression in vitro. Viral particles with retrovirus type-C morphology were found in ultrathin sections of DLC 01 cell pellets. Retroviral particles are spontaneously produced after the 50th cell passage or after induction with 0.5% dimethylsulfoxide. This is the first description of a dog lymphoid cell line spontaneously growing and producing a retrovirus. It was found to share several features in common with feline and murine leukemia viruses.
Find similar cases for your pet
PetCaseFinder finds other peer-reviewed reports of pets with the same symptoms, plus a plain-English summary of what was tried across them.
Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/10450758/