Peer-reviewed veterinary case report
Amino acid changes in feline coronavirus from cat
By Perera, Krishani Dinali et al.·Published in Veterinary microbiology·2019·Department of Diagnostic Medicine and Pathobiology, United States·View original on PubMed →
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Original publication title: Characterization of amino acid substitutions in feline coronavirus 3C-like protease from a cat with feline infectious peritonitis treated with a protease inhibitor.
- Species:
- cat
Plain-English summary
A cat with feline infectious peritonitis (FIP), a serious disease caused by a virus, was treated with an antiviral medication called GC376. Researchers looked at changes in the virus's proteins to see how they affected the treatment's effectiveness. They found that one specific change in the virus made it slightly less sensitive to the medication, but overall, the treatment remained effective over time. This study helps explain why the cat did not develop resistance to the antiviral treatment during its long-term care.
People also search for: cat FIP treatment · feline coronavirus protease inhibitor · GC376 for cats · why is my cat sick with FIP
Abstract
Feline infectious peritonitis (FIP) is a highly fatal disease caused by a virulent feline coronavirus in domestic and wild cats. We have previously reported the synthesis of potent coronavirus 3C-like protease (3CLpro) inhibitors and the efficacy of a protease inhibitor, GC376, in client-owned cats with FIP. In this study, we studied the effect of the amino acid changes in 3CLpro of feline coronavirus from a feline patient who received antiviral treatment for prolonged duration. We generated recombinant 3CLpro containing the identified amino acid changes (N25S, A252S or K260 N) and determined their susceptibility to protease inhibitors in the fluorescence resonance energy transfer assay. The assay showed that N25S in 3CLpro confers a small change (up to 1.68-fold increase in the 50% inhibitory concentration) in susceptibility to GC376, but other amino acid changes do not affect susceptibility. Modelling of 3CLpro carrying the amino acid changes was conducted to probe the structural basis for these findings. The results of this study may explain the observed absence of clinical resistance to the long-term antiviral treatment in the patients.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/31585653/