Peer-reviewed veterinary case report
Detecting epididymitis and testicular lesions in male dogs using PCR
By Camargo-Castañeda, Andrea M et al.·Published in Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·2021·Department of Veterinary Pathobiology, United States·View original on PubMed →
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Original publication title: Characterization of epididymal and testicular histologic lesions and use of immunohistochemistry and PCR on formalin-fixed tissues to detectin male dogs.
- Species:
- dog
Plain-English summary
A group of 8 male dogs with suspected testicular infections were examined for signs of epididymitis (inflammation of the epididymis) and orchitis (inflammation of the testis). The dogs showed varying degrees of epididymitis, and only a few had orchitis. Researchers used special tests, including immunohistochemistry and real-time PCR, to identify the infection in the tissue samples. These tests successfully detected the infection in some of the dogs, suggesting they could be helpful for diagnosing these conditions when other methods fail. Understanding the difference between epididymitis and orchitis can lead to better treatment options and outcomes for affected dogs.
People also search for: dog testicular infection treatment · epididymitis in dogs symptoms · how to treat orchitis in dogs
Abstract
In male dogs,frequently causes epididymitis, ultimately resulting in testicular atrophy and infertility. Althoughpredominantly affects the epididymis, the misleading term "orchitis" is still commonly used by clinicians. Of additional concern, diagnosis in dogs remains challenging because of variable sensitivity and specificity of serologic assays and fluctuations in bacteremia levels in infected dogs, reducing the sensitivity of blood culture. We describe here the histologic lesions in the scrotal contents of 8 dogs suspected of being infected withand clinically diagnosed with orchitis. We explored the possibility of using immunohistochemistry (IHC) and real-time PCR (rtPCR) in formalin-fixed, paraffin-embedded (FFPE) tissues to detect the presence of. Epididymitis of variable chronicity was identified in all 8 dogs, with only 3 also exhibiting orchitis. Using rtPCR, the presence ofwas identified in 4 of 8 dogs, with 3 of these 4 dogs also positive by IHC. These results suggest that rtPCR and IHC are promising techniques that can be used in FFPE tissues to detectwhen other detection techniques are unavailable. Additionally, accurate recognition of epididymitis rather than orchitis in suspect cases could aid in accurate diagnosis.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/33468036/