Peer-reviewed veterinary case report
Characterization of monoclonal antibodies against canine P-selectin glycoprotein ligand-1 (PSGL-1).
- Journal:
- Veterinary immunology and immunopathology
- Year:
- 2011
- Authors:
- Umeki, Saori et al.
- Affiliation:
- Department of Veterinary Medicine · Japan
Abstract
Thirteen different monoclonal antibodies against canine P-selectin glycoprotein ligand-1 (cPSGL-1) were obtained by immunization of rats with cells of a canine lymphoma cell line (Ema). O-sialoglycoprotein endopeptidase treatment of Ema cells showed that all of these antibodies recognized O-glycosylated peptides of canine PSGL-1. Experiments using deletion or point mutants of cPSGL-1 indicated that these antibodies could be categorized into several groups based on their cPSGL-1 recognition characteristics. These anti-cPSGL-1 monoclonal antibodies will be useful for analysis of the canine P-selectin and PSGL-1 system.
Find similar cases for your pet
PetCaseFinder finds other peer-reviewed reports of pets with the same symptoms, plus a plain-English summary of what was tried across them.
Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/21600666/