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Peer-reviewed veterinary case report

Characterization of nontypeableisolates.

Journal:
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
Year:
2020
Authors:
To, Ho et al.
Affiliation:
Nippon Institute for Biological Science · Japan

Abstract

Twoisolates from clinical cases of porcine pleuropneumonia in Japan were positive in the capsular serovar 15-specific PCR assay, but nontypeable (NT) in the agar gel precipitation (AGP) test. Nucleotide sequence analysis of gene clusters involved in the biosynthesis of capsular polysaccharide (CPS) and lipopolysaccharide O-polysaccharide (O-PS) revealed that both clusters contained transposable element ISofbelonging to the IS30 family. Immunoblot analysis revealed that these 2 isolates could not produce O-PS. We conclude that the ISofcan interfere in the biosynthesis of both CPS and O-PS.

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Original publication: https://pubmed.ncbi.nlm.nih.gov/32517629/