Characterization of the hemolytic activity of.

Abstract

infection causes serious economic losses in the duck industry worldwide. Acute septicemia and high blood bacterial loading ininfected ducks indicate thatmay be able to obtain iron and other nutrients by lysing duck erythrocytes to support its rapid growth and proliferation in the blood. However, so far, little is known about the hemolytic activity ofto duck erythrocytes. In this study, 29 of 52.strains showed hemolytic activity on duck blood agar, whereas all the tested dba(with hemolytic activity on duck blood agar) and dbastrains created pores in the duck red blood cells, with 4.35-9.03% hemolytic activity in a liquid hemolysis assay after incubation for 24 h. The concentrated culture supernatants of all the testedstrains and the extracted outer membrane proteins (OMPs) from dbastrains showed hemolytic activity on duck blood agar. These results, together with the median lethal dose (LD50) of some dbaand dbastrains in ducklings, suggested that there was no direct relationship between the hemolytic capacity ofon duck blood agar and its virulence.