Peer-reviewed veterinary case report
Inflammation and itching genes in dog skin with experimental allergic
By Blubaugh, Amanda et al.·Published in Veterinary Sciences·2024·College of Veterinary Medicine, University of Georgia, Athens, GA 30602, USA, United States·View original on Crossref →
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Original publication title: Characterization of the Pro-Inflammatory and Pruritogenic Transcriptome in Skin Lesions of the Experimental Canine Atopic Acute IgE-Mediated Late Phase Reactions Model and Correlation to Acute Skin Lesions of Human Atopic Dermatitis
- Species:
- dog
Plain-English summary
A group of healthy Beagle dogs developed skin lesions after receiving an injection of antibodies that target IgE, which is involved in allergic reactions. These lesions showed signs of inflammation and itching similar to what is seen in dogs with atopic dermatitis (a common skin allergy). The study found that the skin samples from these dogs had increased levels of certain inflammatory markers and immune signals. This suggests that the skin reactions in dogs can closely resemble those in humans with atopic dermatitis. Understanding these similarities may help improve treatments for skin allergies in pets.
People also search for: dog skin itching treatment · Beagle atopic dermatitis symptoms · dog allergy skin lesions
Abstract
Intradermal injection of anti-immunoglobulin E (IgE) antibodies in dogs grossly and histologically resemble naturally occurring atopic dermatitis (AD). However, the activated inflammatory and pruritic pathways have not been characterized. The objectives of this study were to characterize the inflammatory transcriptome of experimental acute canine IgE-induced lesions and to determine how these correlate to the transcriptome of naturally occurring human and canine acute atopic dermatitis. Biopsies were collected at 6 and 24 h after intradermal injections of anticanine-IgE antibodies to eight healthy male castrated Beagles; healthy and saline-injected skin served as controls. We extracted total RNA from skin biopsies and analyzed transcriptome using RNA-sequencing. Gene expressions of IgE-induced biopsies were compared to that of controls from the same subject (1.5-fold change, p-adjusted value ≤ 0.05). Acute IgE-mediated lesions had a significant upregulation of pro-inflammatory (e.g., LTB, IL-1B, PTX3, CCL2, IL6, IL8, IL18), T helper-(Th)1/IFNγ signal (e.g., STAT-1, OASL, MX-1, CXCL10, IL-12A) and Th2 (e.g., IL4R, IL5, IL13, IL33 and POSTN) genes, as well as Th2 chemokines (CCL17, CCL24). Pathway analysis revealed strong significant upregulation of JAK-STAT, histamine, IL-4 and IL13 signaling. Spearman correlation coefficient for the shared DEGs between canine anti-canine-IgE and human AD samples revealed a significant moderate positive correlation for anti-canine-IgE 6-h samples (r = 0.53) and 24-h samples (r = 0.47). In conclusion, acute canine IgE-mediated skin lesions exhibit a multipolar immunological axis upregulation (Th1, Th2 and Th17) in healthy dogs, resembling acute spontaneous human AD lesions.
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Search related cases →Original publication on Crossref: https://doi.org/10.3390/vetsci11030109