Circulating Glioma Model for Chimeric Antigen Receptor T-Cell Therapy Investigations.

Abstract

Circulating tumor cells in cerebrospinal fluid (CSF) are a significant factor in tumor recurrence and intracranial metastasis in glioma. Many studies are attempting to identify effective strategies to target and eliminate the circulating tumor in CSF. Chimeric antigen receptor (CAR) T-cell therapy has been frequently used for various circulating tumors. However, research is limited due to the lack of a suitable circulating glioma model. Here, a circulating glioma model was developed and applied in CAR T-cell therapy investigations. This model was constructed using the lateral ventricle injection, which is widely used in the local treatment of intracranial disease. The glioma cells were injected into the lateral ventricle to form the intracranial primary tumor. The tumors that were shed from the lateral ventricle were used to indicate the circulating tumor in CSF. The model is easy to build and can be applied in various studies on the CAR T-cell therapy of circulating glioma. As a result, the circulating glioma model provides a unique glioma metastasis model for effective CAR T-cell therapy studies of the circulating tumor.