Peer-reviewed veterinary case report
MicroRNA levels drop after spleen removal in dogs with hemangiosarcoma
By Grimes, Janet A & Schmiedt, Jennifer M·Published in American journal of veterinary research·2026·Department of Small Animal Medicine and Surgery, United States·View original on PubMed →
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Original publication title: Circulating levels of microRNAs 126-5p, 150-5p, 214-3p, and 452-5p decrease following splenectomy in dogs with hemangiosarcoma.
- Species:
- dog
Plain-English summary
A group of dogs with splenic hemangiosarcoma (a type of cancer affecting the spleen) had their blood tested before and after surgery to remove the spleen. Researchers found that certain microRNAs (small molecules that help regulate genes) decreased in the blood after the surgery, which might help in understanding the disease better. This could lead to new ways to diagnose hemangiosarcoma without invasive procedures. The dogs were monitored for changes in these microRNAs, which could be important for future treatments and diagnostics.
People also search for: dog hemangiosarcoma symptoms · splenectomy recovery in dogs · microRNA testing for dog cancer
Abstract
OBJECTIVE: To identify microRNAs (miRNAs) differentially expressed in the serum of dogs with splenic hemangiosarcoma between preoperative and postoperative samples. METHODS: RNA was extracted from serum collected from dogs with hemangiosarcoma preoperatively, 24 to 48 hours postoperatively, and 10 to 30 days postoperatively. Quantitative reverse transcription PCR was performed with differential expression analysis between sample time points for miRNA (miR)-126-5p, miR-150-5p, miR-203a-3p, miR-214-3p, miR-452-5p, miR-494-3p, miR-497-5p, and miR-543 using miR-16-5p and miR-93-5p as controls. RESULTS: Serum from 17 dogs with splenic hemangiosarcoma (13 with stage II disease, 4 with stage I disease) was collected preoperatively (n = 17), 24 to 48 hours postoperatively (17), and 10 to 30 days postoperatively (5). MicroRNA-126-5p expression decreased at both postoperative time points compared to the preoperative time point. MicroRNA-150-5p and miR-214-3p expression decreased between the preoperative and 24- to 48-hour postoperative time point, although neither had a significant change in expression between the preoperative and the 10- to 30-day postoperative time point. MicroRNA-452-5p had decreased expression at the 10- to 30-day postoperative time point compared to the preoperative time point. There was no change in expression of miR-203a-3p, miR-494-3p, miR-497-5p, or miR-543 between any time points. CONCLUSIONS: MiR-126-5p, miR-150-5p, miR-214-3p, and miR-452-5p all showed a decrease in expression at at least one timepoint following splenectomy. CLINICAL RELEVANCE: These miRNAs have been previously shown to have roles in angiogenesis and pathways known to be involved in the pathogenesis of hemangiosarcoma and may be prospective targets for a minimally invasive diagnostic panel for the presence of splenic hemangiosarcoma.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/41289681/