Peer-reviewed veterinary case report
Accuracy of 3 quick antibody tests for Brucella canis in dogs
By Atkins, Callum et al.·Published in Journal of veterinary internal medicine·2026·Hospital for Small Animals, United Kingdom·View original on PubMed →
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Original publication title: Clinical performance of 3 commercially available point-of-care antibody tests for Brucella canis in dogs.
- Species:
- dog
Plain-English summary
A group of dogs was tested for Brucella canis, a bacteria that can cause brucellosis, which may not show obvious symptoms. Researchers evaluated three different quick tests to see how well they could detect antibodies against this bacteria. Two of the tests, the Anigen Rapid C.Brucella Ab and the FASTest BRUCELLA canis, performed well, accurately identifying dogs with the infection most of the time. However, the third test did not work effectively. These findings suggest that the first two tests could help vets quickly rule out brucellosis in dogs showing signs of illness, but further testing may still be needed for confirmation.
People also search for: dog brucellosis symptoms · Brucella canis test accuracy · quick tests for dog infections
Abstract
BACKGROUND: Brucellosis in dogs, caused by Brucella canis, is a zoonotic disease often underdiagnosed because of diagnostic challenges and subclinical infection. Bacterial culture, the reference standard, is impractical and has limited sensitivity because of intermittent or low-grade bacteremia. Accurate point-of-care (POC) testing could facilitate timely management and infection control. HYPOTHESIS/OBJECTIVES: Evaluate the diagnostic performance of 3 POC lateral flow assays for detection of B. canis antibodies in dogs, using the Canine Brucella Multiplex (CBM) assay as the reference serologic assay. ANIMALS: Fifty serum samples from 50 client-owned dogs submitted for B. canis serology. METHODS: Samples were classified as CBM-positive (n = 10; culture-positive dogs) or CBM-negative (n = 40; healthy dogs undergoing export testing). Assays were run in duplicate and independently interpreted by 2 observers. Positive percent agreement (PPA), negative percent agreement (NPA), and inter-reader agreement were calculated relative to CBM. RESULTS: The Anigen Rapid C.Brucella Ab assay demonstrated 90.0% PPA (95% CI, 55.5-99.8) and 100% NPA (95% CI, 91.1-100). The FASTest® BRUCELLA canis assay demonstrated 100% PPA (95% CI, 69.1-100) and 92.5% NPA (95% CI, 79.6-98.4). The Canine Brucella LPS Antibody Rapid Test demonstrated 0% PPA (95% CI, 0-30.9) and 97.5% NPA (95% CI, 86.8-99.9). Inter-reader agreement was almost perfect for all assays (κ = 0.81-1.00). CONCLUSIONS AND CLINICAL IMPORTANCE: The Anigen Rapid C.Brucella Ab and FASTest® BRUCELLA canis assays showed good agreement with the CBM assay and may be useful rule out tests in dogs with clinical signs compatible with brucellosis. In low prevalence settings, positive results require confirmatory testing, and false negatives remain possible.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/42060494/