Clinical Value of MiR-191-5p in Patients with Sepsis and Its Regulatory Role in Sepsis-Related Left Ventricular Systolic Dysfunction.

Abstract

In this study, the expression and clinical value of miR-191-5p in sepsis and sepsis-induced left ventricular systolic dysfunction are explored, and the effects of miR-191-5p on inflammation and cardiac function in rats are examined by establishing a septic rat model.The expression level of serum miR-191-5p was detected via reverse transcription quantitative polymerase chain reaction. To evaluate the diagnostic efficacy of miR-191-5p in sepsis, the receiver operator characteristic curve was established. To evaluate the relationship between the expression level of miR-191-5p and the clinical biochemical indicators, Pearson analysis was employed. Multivariate logistic regression was utilized to analyze the risk factors of cardiac dysfunction. The rat model was reproduced via cecal ligation and puncture. FDP-1 HRV-BRS analysis system was utilized to detect the cardiac function. To determine the myocardial enzymes and cytokines, blood samples from rats were collected.Compared with healthy individuals. the expression of miR-191-5p in the serum of sepsis patients was lower, and the miR-191-5p level in the serum of patients with cardiac dysfunction was lower than in that of those with normal cardiac function. MiR-191-5p demonstrated good accuracy in distinguishing cardiac dysfunction from normal cardiac function. Decreased levels of miR-191-5p are a risk factor for developing normal cardiac function in sepsis into cardiac dysfunction. In septic rats, upregulating the miR-191-5p expression by injecting miR-191-5p agomir can significantly enhance cardiac function and inhibit the inflammatory response.MiR-191-5p has shown clinical value in distinguishing patients with sepsis associated with left ventricular systolic dysfunction and has the potential as a diagnostic marker for sepsis.