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Peer-reviewed veterinary case report

Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD.

Journal:
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]
Year:
2013
Authors:
Leal, C A G et al.
Affiliation:
Laborat&#xf3 · Brazil

Abstract

The aims of this study were to standard and optimize a qPCR protocol with FAM-BHQ1 probe, and to compare its sensitivity against TaqMan qPCR and PCR methods to diagnose shrimp WSD. The FAM-BHQ1 qPCR presented higher clinical sensitivity and showed to be a robust alternative to detect WSSV in clinical samples.

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Original publication: https://pubmed.ncbi.nlm.nih.gov/24516428/